Peptides with Fluorescent Labels

 

Different fluorescent dyes can be attached to peptides in different locations to label them. For a wide variety of imaging and diagnostic applications, fluorescent tags or probes are perfect. Applications for fluorescent peptide synthesis include localization investigations, peptide-protein interactions, enzyme activity assays, in vivo biomedical imaging research, and the creation of new disease models. Fluorescence microscopy and other fluorescence visualization methods can be used to see fluorochrome-conjugated peptides. An indirect method of labeling is to use an amino acid that has been biotinylated. The acceptor and quencher components may be joined inside or outside. For longer sequences, the internal connection is advised.

Studies on protein binding and localization, as well as in vivo biomedical imaging, typically make use of fluorescently tagged peptides. Fluorescence microscopy and other fluorescence visualization methods can be used to see fluorochrome-conjugated peptides. There are several different fluorescent labels. These labels can be inserted into your peptide at any desired location. Different fluorescent dyes can be attached to peptides in different locations to label them.

To meet your needs, we provide a variety of luminous labeling choices. The fluorescein derivative is a carboxyfluorescein that exhibits emission. The fluorescent dye is most frequently used to bind to peptides. Additionally, FAM is utilized with the majority of fluorescence-detecting tools. It can be linked to either the carboxy- or amino-terminal and is most frequently utilized in the pH range of 7.5 to 8.5. Nucleotides and nucleic acids are labeled and sequenced using this fluorescein. Rhodamine derivative stands for tetramethylrhodamine. It is most frequently utilized to make bioconjugates, like fluorescent antibodies used in cellular imaging and immunochemistry research. However, DNA sequencing and oligonucleotide tagging are other applications.

The biotech industry makes use of cyanine dyes. In fact, cyanine dyes are well-known for use in immunoassays, research in proteomics, transcriptomics, and genomic hybridization. Peptides, proteins, and antibodies are labeled with cyanine dyes for fluorescence detection methods like flow cytometry, microscopy, and microarrays.