The Advantages of Using Solid-Phase Peptide Synthesis
Organic chemists around the world have been using amide condensation processes for decades to remarkable success. As the demand for synthetic analogues of bioactive peptides increased in the middle of the twentieth century, several peptide synthesis companies quickly realised that the "normal" solution phase techniques would not be sufficient. It's difficult and time-consuming to synthesise peptides in the solution phase since the coupling reactions are protracted and recrystallization or column chromatography is required after each amino acid coupling. Removal of Unwanted Soluble Compounds Is Easy So-called solid phase polypeptides are those in which a polymer substrate is linked to the initial amino acids of the sequence. The polymer makes any molecules connected to it insoluble, making it easier to distinguish them from soluble ones that can be washed off. The necessary chemical reactions between free-floating amino acids and the existing chain are made easi...