Our Antibody Production Process

 

As a leading antibody production company, we strive not only at providing best-in-class facilities but also a broad range of solutions to prudently adapt to each project stipulation. Our ardent team is also willing to share its long experience with its clients. Significance, we are currently developing a newer interactive form to guide you to describe the most relevant antibody production policy for your project.

Why is appropriate antigen design so crucial for the production of quality antibodies?

Drawing from our widespread experience in antibody development, we know antibody production services can make or break any project. To make the most of your chances of success, we continuously consider the native conformation of your antigen & the final intended application of your antibody.

For this, we continuously invest in antigen design divergence:

Proteins: these classic antigens continue to be the most significant approach to antibody generation. The use of entire recombinant proteins guarantees your antibodies recognize a single antigen. But this method also increases the risks of making antibodies with lower selectivity and prone to binding preserved domains shared by numerous different proteins. Here, we can produce proteins in a diversity of recombinant systems.

Mammalian cells: Produce your protein antigens in CHO or as the best mammalian hosts on the market

Bacterial cells: Make your antigens in Escherichia coli for higher productivity

Insect cells: Produce your protein antigens in our insect cells for improved protein folding

DNA: DNA immunization is used for difficult proteins or transmembrane proteins. The main benefit of DNA immunization remains the possibility to direct the antigen to its native conformation.

Peptides: using peptides as an antigen favours low cross-reactivity & higher specificity. But they are often less immunogenic due to their smaller size.

Designing a peptide antigen for antibody manufacture requires considering numerous factors:

Avoid peptides comprising sequences that are conserved among numerous protein families

Favour flexible & solvent-exposed sequences which will remain accessible through the process of antibody generation

Favour hydrophilic arrangements which are more prone to be solvent-exposed than hydrophobic constructions, generally hidden in the structure. They are also cooler to solubilize before injection.