Pegylated Peptide Sequences help Reduce Hydrophobic Peptide Aggreagtion.

 

Genosphere Biotechnologies specializes in the production of hydrophobic peptide sequences and long peptides by carefuly planning the synthesis routes and incorporating many processes to overcome difficulties in the manufacturing process. While we have good reputation for being able to produce difficult peptides, it is always best to avoid known areas of concern when possible. For example, an overly hydrophobic peptide sequence, even when we are able to be produced it, generally cause production delays, increased manufacturing costs and difficulty in future scale up. Our expert peptide chemists specialize in sequence design that optimizes stability, solubility, and manufacturability of scale up, while maintaining the core active region of the peptide unchanged.

 While the below tips are meant to help solubility, stability and future scale up, we understand changing a peptides amino acid composition isn’t always possible. In these cases, our expert peptide chemists will optimize a custom protocol to ensure a timely, cost-effective delivery, even when challenges present themselves.

 Avoiding an excessively hydrophobic peptide sequence permits usually higher yields, better chromatographic separation and thus easier purification, decreased leadtimes and lower costs for future scale-up.  As a rule of thumb, a sequence that includes over 50% hydrophobic residues (A,V,L,I,M,F,W,P) is considered a hydrophobic peptide.  One may try incorporating as many hydrophilic/charged residues (K,R,H,D,E) as the design will allow.  While most encountered solubility issues may be overcome with the use of alternative solvents and sequence specific pH adjustments, a highly hydrophobic peptide may cause low yields and inconsistency in the hands of the scientist performing downstream laboratory experimental work.

 PEG derivatives for peptide synthesis are used to improve the solubility of hydrophobic peptide sequences.  Pegylated peptide enhances the bio-availability of bio-active sequences, and to shields the peptide moiety from triggering an immune response. Polyethyleneglycol (PEG) derivatives have an amphiphilic nature.

 Genosphere Biotechnologies can prepare your pegylated peptide at a variety of sites on the peptide chain.  Genosphere Biotechnologies will deliver your pegylated peptide with a certificate of analysis as a regular end-product.

 

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