Antibody
Production Services
Genosphere Biotechnologies offers complete polyclonal antibody
production services packages, including peptide or protein antigen synthesis,
choice of hosts immunization, antibody purification, and quality control analyses.
Our polyclonal antibody
production services are designed to meet a variety of experimental
requirements. For example, depending of the planned experiments, antisera or
purified antibodies can be provided. Multiple purification methods are
available, including protein A and antigen affinity purification. Purification of the antisera is used to
enrich the antibody activities.
Genosphere Biotechnologies has long experience in successful polyclonal
antibody development and guarantees an ELISA titer above 1:100,000. We offer multiple packages that can fit
various customer needs. Our featured development packages can guarantee the
delivery of fully-validated rabbit, guinea pig, rat, or mouse polyclonal antibodies within 70 days.
Our polyclonal antibody development services are based on years of
process optimization and a strong reputation for reliable antibody production
services. We have been a preferred partner to various institutions worldwide
for antibody development and antibody production services for over 25 years.
Antibody Production
Services Highlights
• Guaranteed ELISA titer >1:50,000.
• Proprietary peptide design tools and
high-efficiency conjugation ensure a >95% success rate of peptide
immunization.
• Integrated antigen preparation: from gene synthesis to protein antigen
expression and purification
Monoclonal
Antibody Development
Mouse monoclonal
antibodies have broad applications as therapeutics, diagnostic tools, and
research reagents. Genosphere Biotechnologies provides comprehensive mouse
monoclonal antibody development services based on our well-established
hybridoma platform. We provide complete support for all steps of mouse
monoclonal antibody development, from antigen preparation to animal
immunization, fusion and screening, subcloning, and antibody production, which
allows the identification of clones with the desirable qualities.
We provide free
consultation for target antigen design and expression, and our experienced
project managers and scientists will support you directly throughout the
process.
Since the
Nobel-winning prize pioneer work in 1975 on hybridoma technique the monoclonal
antibody development and manufacturing process has been refined. In a nutshell, the generation of hybridomas
involves immunising mice against a specific epitope on an antigen and obtaining
the B-lymphocytes from the spleen of the animals. The B-lymphocytes are then
fused with an immortal myeloma cell line lacking the
hypoxanthine-guanine-phosphoribosyltransferase (HGPRT) gene. These hybridoma
cells are further cultured in vitro in selective medium (i.e.
hypoxanthine-aminopterin-thymidine +) where only the hybridomas -that is the
fusion between the primary B-lymphocytes and myeloma cells- can survive as they
have inherited immortality from the myeloma cells and selective-resistance from
the primary B-lymphocytes. The initial
culture of hybridomas contains a pool of antibodies derived from many different
primary B-lymphocyte clones, each secreting its own individual specific
antibody into the culture medium hence, at this point, the antibodies are still
polyclonals. Each individual clone is separated by dilution and the supernatant
screened for the targeted specific antibody.
These cells are then characterized and cloned to allow production over
time.
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